Evaluation of the efficiency of some Natural and chemical Substances in inhibiting the Biofilm of Enterobacter cloacae and Stimulating potato plant Resistance in the field

Abstract

Soft rot disease caused by different species of bacteria Enterobacter cloacae, Pectobacterium carotovorum, Dickeya is one of the most important diseases that destroy most fruits and vegetables globally. Due to the widespread use of chemical pesticides before harvesting, which led to environmental pollution which affects human health, amino acids and seaweed extract were used as natural materials and some chemicals were used to enhance plant defences against the pathogen.The results of the cell membrane revealed that the concentration of 0.5 for each of the amino acids, seaweed extract and copper 0.25 led to inhibition and biofilm formation, reaching <0.12 (0.11), <0.12 (0.10), respectively. In the field, the effect of applying the interactions A.a + Sef + cop + E.c significantly impacted the level of POD enzyme activity in plants treated in the preventive and therapeutic experiment, as it reached 33.7, 29.5 g / fresh weight. The overlap between them amounted to 31.6 g / fresh weight, compared to the control treatments, which amounted to (20.2, 18.3, 19.3), respectively.The combination A.a + Sef + cop + E.c significantly increased the activity of the PAL enzyme, as the enzyme levels reached 40.40 and 39.32 mg/fresh weight for the preventive and therapeutic methods, respectively, compared with the control treatment, which amounted to 21.25 and 20.95 mg/fresh weight. The effect of overlapping the treatment (A.a + Sef + cop) and the preventive and therapeutic experiments was 37.71 mg / fresh weight, with a significant difference from the control treatment, which amounted to 21.10 mg / fresh weight.The treatment of amino acids + E.c influenced increasing the total phenols in the plant, reaching 181.6 in the preventive experiment compared to the control treatment, which amounted to 153.4. Amina and spraying with seaweed extract led to eliminating bacterial soft rot caused by bacteria E.c and prolonging the storage process.