Effect of plant growth regulators and type of media on multiplication and rooting of peach rootstock (Prunus persica L.) Garnem In vitro

Abstract

Experiments were conducted in one of the private laboratories of Al-Fahama / Baghdad for August 2020 to June 2022 period. This study aimed to achieve an appropriate protocol for propagation the roortstock of peach Garnem (Prunus persica cv. Garnem) In vitro. The shoot tips and nodes of this rootstock were used as explant for the stages of emergence it was sterilized with several levels of sodium hypochlorite NaOCl (0.5 and 1%) for three periods of 15, 20 and 25 minutes cultured on MS media with free hormone, after which an experiment was conducted to find out the appropriate medium for multiplication, so it was used WPM and MS media added to it the best concentration of Kin. which he reached from previous experiments by interfering with concentrations of BA(0, 1 and 2 mg l^-1) in order to multiply the shoots. Use concentrations of IBA for rooting shoots are 0, 0.5, 1, 2 and 3 mg l^-1 added to media1/2 WPM and WPM. The results of the study were analyzed statistically as factorial experiments using a completely randomized design (CRD), and the statistical program SAS (2003) was used to analyze the results, and the averages of the coefficients were compared according to Duncan's multiple test in the probability level of 0.05. The results showed efficient use NaOCl in the sterilization of peach rootstock shoot tip Garnem, the best multiplication was achieved when the shoot tips were grown on WPM media added to it 1 and 2 mg l^-1BA. also, the highest number and length of shoots were obtained at 1 mg l^-1. It gave shoots planted on the 1/2WPM to which a concentration of 2 mg l^-1 was added IBA the highest rooting percentage, the highest number of roots, and the highest length of roots.