The effect of the multiple genotypes of the MYOSTATIN gene on the reproductive characteristics of the Iraqi brown local chickens

Abstract

     The experimental flock consisted of 101 laying hens attributed to the father and mother from the local Iraqi chickens at the age of 74 weeks / the third generation belonging to the fields of the Poultry Research Station in the Agricultural Research Department / Ministry of Agriculture The chosen one is based on the characteristic of high egg production production rate is more than 60%, and the experiment aims to determine the multiple genetic features of the myostatin gene (MSTIN) and its contribution to affecting the average body weight in a sample of Iraqi brown local chickensAfter taking blood samples from the pterygoid vein and extracting DNA with a concentration of 98% and a purity of 19.8, PCR-Product technique was used to multiply the segment of the Myostatin gene, and sent to the Republic of South Korea, Macrogen Corporation-Korea, and using the Sanger and Coulson method, the genetic phenotypes of genetic mutations were determined and their nitrogenous bases were sequenced on the genome with high accuracy, The percentages of individuals carrying it contributed to the variance of genetic frequency and chi-square values (24.8, 58.5 and 37.6 for mutations, respectively) and their incompatibility with the Hardy-Weinberg rule. The hybrid genotypes (CG, AB, and TC) showed significant p<0.05 in the weekly egg production rate throughout the productive periods, While there were no significant differences in the average weight of the first egg (36.32, 36.37 and 36.32 g for mutations, respectively), as well as no significant difference in the measures of egg quality (white and yolk weight and their percentages) among all members of the local brown chicken under study.