Effect of silver nanoparticles and NaCl salt on the growth and content of some active compounds of Allium sativum L. callus ex vivo

Abstract

Abstract

    The experiment was carried out in the palm propagation unit at the Faculty of Agriculture / University of Kufa, in 2020-2021. The study involved the effect of two factors: First, different levels of AgNPs (A1= 0, A2= 1, andA3= 2 mg.L^-1), and second factor : different levels of sodium chloride salt  (B1= 0, B2= 25 and B3= 50 mmol.L^-1) On the Biomass weight and growth rate of the callus as well as their effect on the content of the compounds S-allylcysteine, Allyl methyl trisulfide and Diallyltrisulfide In the callus cells of garlic plants, ex vivo. A completely randomized design factorial experiment (C., R., D.). AgNPs Showed superiority in the Biomass weight and growth rate. The focus  (1mg.l^-1) Record the highest rate Biomass weight and growth rate reached (468.4 mg and 13.16 mg.day^-1) sequentially. As well, theer are Positive increase between different levels of sodium chloride salt. focus (25 mmol.l^-1) was the highest in the Biomass weight and growth rate reached (462.8 mg and 12.96 mg.day^-1) sequentially. AgNPs Showed superiority in the secondary metabolites. The focus (2mg.l^-1) Record the highest rate in S-allylcysteine, Allyl methyl trisulfide and Diallyltrisulfide content (168.28, 225.41and 533.14µg.ml^-1) sequentially. As well, theer are Positive increase between concentrations of sodium chloride salt. The focus (50 mmol.l^-1) Record the highest rate in S-allylcysteine, Allyl methyl trisulfide and Diallyltrisulfide content (98.26, 201.85 and 510.23 µg.ml^-1) sequentially. whereas, the Overlap treatment (2 mg.l^-1 of AgNPs and 50 mmol.l^-1 of NaCl) recorded Positive increase in S-allylcysteine, Allyl methyl trisulfide and Diallyltrisulfide content (233.84, 285.31 and 608.44 µg.ml^-1) sequentially.