Optimal conditions for vitamin B12 production from Lactobacillus rhamnosus

Abstract

This research was conducted to study the effect of some nutrients on the growth and production of vitamin B₁₂ by Lactobacillus genus L.rhamnosus, when their ability to produce vitamin B₁₂ was tested under different conditions. The results indicate that a pH of 7 is optimal for vitamin B₁₂ production by the bacteria L.rhamnosus and it was found that the optimum incubation temperature for the production of vitamin B₁₂ ranged between 30 - 35 °C, with regard to the effect of the incubation period on the biological activity and productivity of vitamin B₁₂. The results showed that 3 days was the best in the production of vitamin B₁₂. The results also showed that adding the lowest concentration of cobalt chloride gave a high productivity of the vitamin compared to the highest added concentrations and that the best carbon source was sucrose among the three carbon sources that included glucose, fructose, and sucrose, and the results of the study showed that the best nitrogen source It is was peptone compared to yeast extract, which gave the highest production of the vitamin B₁₂.