Effects of some plant growth regulators and different culture media on in vitro shoot multiplication of Photinia (Photinia x fraseri)

Abstract

The present study was carried out to examine the influence of some plant growth regulators and different culture media on in vitro culture of Photinia. Lateral buds were used as explants and surface sterilized by dipping explants in a solution containing 0.1% (w/v) mercury chloride (HgCl₂) for 5 min. to determine the most suitable medium and plant growth regulators for shoot multiplication, explants were excised and cultured in different media (Murashige and Skoog (18) (MS); White (30); Gamborg (12) (B5); and Driver and Kuniyuki walnut (11)(DKW)  containing various concentrations of Kinetin (Kin) (1, 2 and 3 mgl^-1) and Indole-3-butyric acid (IBA) (0.0, 0.2, and 0.4 mgl^-1) and their interactions. The best number of shoots per explant (3.2 shoots/ explant) was produced on MS plus 2 mgl^-1 of Kin and IBA at 0.2 mgl^-1 and the highest number of leaves per explant (12.20 leaves/ explant) was produced on DKW at 2mgl^-1 of Kin and IBA at 0.2mgl^-1, this increases was significantly which compared to all treatments except this treatment containing (DKW + Kin 1, 2 mgl^-1+ 0.4mgl^-1 IBA). DKW medium was recorded the highest average of shoots length/ explant when medium containing both Kin at 2 mgl^-1 with 0.2 mgl^-1 of IBA as compared with others combinations.