Processing browning and increasing multiplication and rotting of Peach rootstock cv. Garnem by using antioxidants and plant growth regulators in vitro

Abstract

The study aimed to find an accurate propagation program for the rootstock of peach Prunus persica cv. Garnem. The apical shoots and nodes of this rootstock were used as explants for the stages of initiation and multiplication, and they were planted on MS medium after adding different concentrations of Active Charcoal (AC), Polyvinyl Pyrrolidone (PVP), and Benzyl adenine (BA). The shoots were planted for the purpose of rooting on MS medium containing different concentrations of salts (1/4 MS, 1/2 MS and full MS salts) with different concentrations of IBA. In the establishment phase, the apical shoots grown on medium supplied with AC were given the highest response rate, the highest average length of plantlet, and the highest number of leaves. The best multiplication of explants in terms of the number of shoots and their length were obtained when cultivated on a medium supplemented with BA at 1 and 2 mg.L^-1, whereas the application of BA at 1 mg. L-1 to the cultivated nodes gave the highest number of leaves. The best rooting percent was obtained when plantlets were s treated with 2 mg. L^-1 was applied to 1/4 MS medium. The highest number of roots was obtained from plantlets grown in 1/2 MS medium treated with IBA at 3 mg.L^-1, whereas the longest root was obtained from plantlets grown in 1/2 MS medium treated with 1 mg.L^-1IBA.