Chemical Analysis of Euphorbia denticulata Lam. and Study of extracted oil on Cytotoxicity and anti-tumor potential on AMGM-5 and HBL 100 cell lines

Abstract

Study have been conducted on the cytotoxicity of Euphorbia denticulate oil. In The normal human breast cell line HBL 100 and human brain cancer cell line AMGM-5 Depending on the MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay method Following the application of various oil concentrations (0.01, 0.05, 0.1, 0.5, 1, 5) μg \ ml to the cells, the current study was able to determine the The Half maximal inhibitory concentration( IC50) value in AMGM-5 to be 0.21505%, while the normal cell line HBL100 revealed an IC₅₀  value of 0.18688%). the MTT assay test findings suggested a suppression of the proliferation of cancer cells. The AMGM-5cell lines changed cellularly as a result of the oil  Following a 48-hour course of therapy, the degeneration showed varying degrees of atrophy, swelling, and breakdown. Ultimately, the cells completely disintegrated, resulting in the formation of necrotic regions devoid of cells. Cultures of cells: When the cells were exposed to high oil concentrations, these alterations worsened