Synthesis, Characterization of some Metallic Complexes and Exploring the Anticancer Activity of Au(III) Complex with Newly Ligand drived from xanthine compounds

Abstract

The new Azo-Schiff ligand8-((1E)-(2-(((4-hydroxy-3-methoxybenzylidene)amino)methyl)phenyl)diazenyl)-1,3,7-trimethyl-3,7-dihydro-1H-purine-2,6-dione   was prepared from Ortho-aminobenzylamine azoate and reaction with  Caffeine, followed by the condensation of the resulting compound withVanillin . Different analytical and characterization techniques including (FT-IR, UV-Vis. Spectroscopy, GC-mass, ¹HNMR and C.H.N elemental analysis) in the investigation of newly prepared ligand. A series of novel solid metal complexes of this ligand with Cu (II),Ag(I) and Au(Ⅲ) were prepared and all complexes were characterization by techniques above, excluding the mass and the ¹H-NMR spectroscopy of some prepared solid metal complexes and the use of flame atomic absorption spectroscopy to determine the percentages of metal ions in the prepared complexes and molar conductivity of the metal complexes dissolved in DMSO at 1 × 10^-3 M concentration laboratory temperature. The results of this studies showed that the coordination sites for the new Azo-Schiff base ligand with Cu (II), Ag (I)  were to be through nitrogen of the Caffeine ring, ,the nitrogen of azo group and the nitrogen of azomethine group . The Electronic spectral and magnetic measurement data predict octahedral structure of the Cu (II),tetrahedral of the  Ag (I)  complexe while it has a square planar shape with the Au(III) ion. All complexes have electrolytic properties . Significant antiproliferative activity was seen in it against human lung cancer (A549) and normal cell lines (HDFn), as evidenced by the IC50 values. This study could contribute to the development of pharmaceutical preparations.