Molecular Evaluation of the Effect of Using Chitosan Nanopolymers on Loaded Marine Fish Acellular Dermal Matrices in Reconstruction of Induced Ventral Hernias in Bucks

Abstract

The aim of present study was to compare between the effectiveness of marine fish acellular dermal matrices (ADM) and Chitosan Nanopolymers (ChNPs) loaded on fish ADM in the reconstruction of large abdominal wall hernias in Iraqi bucks. Molecular evaluations were conducted using Real Time-quantification Polymerase Chain Reaction (RT-qPCR) to measure the levels of basic fibroblast growth factor (b-FGF) and vascular endothelial growth factor (VEGF) genes during the healing process. Ventro-lateral abdominal wall hernias (6x6cm) were induced in 18 bucks. Thirty days post-induction of hernia, the bucks were divided into two groups: Group A, control group treated with fish ADM and group B, ChNPs group which was treated with fish ADM loaded with ChNPs. Evaluations were performed at 2, 8, and16 weeks post-treatment. Molecular analysis revealed significantly higher b-FGF and VEGF gene expression in the ChNPs group compared to control group, particularly at week 16^th week post-treatment.