Gene study of Oval and OCX-32 in laying and non-laying hens

Abstract

Specific genes tightly control the laying hen’s oviduct's egg development process, and each section of the oviduct adds a distinct element to the final product. The aim of the study was to study the oval, and ocx32 genes in both laying and non-laying hens. A total of 38 adult hens were split into two groups; laying and non-laying hens were involved in this study. From the magnum, isthmus and uterus tissues RNA was isolated and transcripted to cDNA for gene expression study. In comparison to other places, the data indicated that oval was significantly increased in laying hens at the Magnum region as opposed to non-laying chickens. Compared to non-laying hens, laying hens' uterine and isthmus regions showed significantly higher levels of ocx32. Gene ontology study revealed that oval gene plays a key role in matrix protein regulation while ocx32 play a key role in eggshell quality via calcium and lipid metabolism. Moreover, gene analysis showed negative regulation of endopeptidase activity, regulation of proteolysis, and regulates the mineralization process and contributes to the structural integrity and antimicrobial properties of the eggshell. In conclusion, a common method for understanding high throughput molecular data and generating hypotheses about the biological mechanisms underlying investigations is Gene Ontology enrichment analysis. by providing a shared vocabulary for describing how genes function.