Tumour necrosis factor-α (TNF-α) in mycotic aborted placenta : an evaluation by Immunohistochemistry

Abstract

the present study aims to study histopathological changes in the mycotic aborted and normal placenta of sheep ,in addition to study the levels of TNF-a in mycotic aborted and normal placenta by immunohistochimistry assay (IHC) Seventy five , samples of aborted placenta and twenty five of normal placenta were collected , during November 2017 to May 2018 from certain regions of AL-Najaf city . Placental samples were fixed in 10% neutral buffer formalin for 72 hrs for histopathological examination, paraffin embedded tissue belong to mycotic aborted and normal group were used for assessment of TNF alpha by immmunohistopathological study was carried out to determine TNF protein in the placenta sample. Histopathological examination showed suppurative necrotized placentitis, in addition to congestion of the blood vessels and fibrin deposition in the intera-villius space as well as calcium deposition in necrotized the necrotic area and fibrosis of stromal area in certain chorionic villi and sloughing and desquamation of syncytotrophoblast. Result of IHC of TNF alpha protein showed significant elevation in TNF alpha protein in inflammatory cell (macrophage) of aborted sheep placenta (increasing of intensity and score), while no changes could be observed for pro-inflammatory molecules in the control sample (normal delivery).