Potentiate the anticancer effect of Methotrexate by Zingiber officinale Roscoe extract

Abstract

This project considered an explorer study for the effect of crude extracts of Zingiber officinale on two cell lines, one was cancer cell lines, and the other was normal cell lines as well as study its potentiating effect on anti-tumor activity of methotrexate.

    The aqueous extract was prepared from dried rhizome of Z. officinale yields of extraction was 10%. Study the cytotoxic activity of the prepared extract on cancer cell lines mouse breast 4TI and normal cell line (human embryo kidney HEK293 in ten concentrations of two fold dilution begin with 4000 μg/ml until 7.81 μg/ml for extract and starting with 500 μg/ml until 0.976 μg/ml for methotrxate, at 48 hours of exposure.   After the end of exposure of each cell lines to extract, the microtitration plates were washed and treated by crystal violet stain and the optical density of the plate wells were read by the ELISA reader at wave length of 495 nm used as parameter for the viability of cell line. The results showed that the aqueous extract exhibited significant concentration-dependent, specific inhibitory effects on 4TI malignant cell line in comparison with control and the cytotoxic effect increased according to the concentration. The extracts produced little cytotoxic effects on normal HEK293 cell line, when compared to mthotrexate which produced inhibitory effects on normal HEK293 cell line. In this study was showed that the cytotoxic effect of Methotrexat on 4TI cell line enhanced when given with Z. officinale extract together.