Molecular Detection of AmpC Family Genes Encoding Antibiotic Resistance among Escherichia coli isolated from Patients with Urinary Tract Infection (UTI) in Najaf Hospitals

Abstract

    One hundred and thirty significant bacteriuria were detected. The study showed higher incidence of UTI in females (73.08%) than males (26.9%). The E. coli isolates which grown on  culture of all the 130 urine samples with significant bacteriauria were  60 ( 46.2 %) . The isolates which were resistant to ampicillin and amoxicillin 53(88.3%) . The ability of E. coli isolates to AmpC production were tested; the results have revealed that 23 (43.4%) isolates produce AmpC β-lactamase. PCR amplification results have shown that eighteen E. coli isolates possess AmpC β- lactamase gene. The results in this study showed 8 (44.4%) had bla_FOX gene and 7(38.9%) had bla_CIT while  5(27.8%) of E. coli isolates had bla_DHA and 9(50%) had bla_EBC gene.