Genotyping of Candida albicans Isolated from broilers by 25S rDNA Analysis

Abstract

    Candida albicans one of the most fungi which isolated from clinical samples that cause many problems to humans, animals and birds. Candidiasis is an opportunistic endogenous mycosis in that perturbance of the microflora or other debilitation of the host.  So, this study come to focused on genotyping and antifungal susceptibility profile to Candida albicans isolated from broilers.

Ten isolates of Candida albicans were obtained from crop of broilers, samples was collected from poultry diseases lab. in Najaf  veterinary hospital of different cases of enteritis broilers. All isolates were identified morphologically and biochemically by Hicandida identification kit and genotyped according to 25s rDNA of transposable intron I region of chromosome R.

    The results  showed that all isolates belonged to the genotype A with amplification product (450 bp). Antifungal sensitivity profile by disc diffusion method showed no resistant to nystatin, ketoconazole and micanazole while three isolates (30%) showed resistant to fluconazole.