Effect of alcoholic extracts ( Cuminum cyminum,Thymus vulgaris and Menthe pulegium) and tri mixed and di mixed extracts on enterobactericea (E. coli , Brucella abortus, Salmonella entridius) growth in vitro

Abstract

    The present study aimed to determine the effect of alcoholic extracts of ( Cuminum cyminum,Thymus vulgaris and Menthe pulegium) activity against some of enterobactericea of gram negative stain  (E. coli , Brucella  abortus Salmonella entridius) ,at a varying concentration of 10 mg/ml by using agar well diffusion methods.                                             

    Different chemical detection methods indicate that Cuminum cyminum contained Flavonoids, Thumus vulgaris contained Reins and Saponins while Menthe pulegium contained Flavonoids.                                                                       

    The result showed that Brucella  abortus more sensitive for antimicrobial activity alcoholic extract of  Menthe pulegium and equal to tri mixed alcoholic extracts and di mixed, one of( Cuminum cyminum and Menthe pulegium), Cuminum cyminum alcoholic extract and  Thymus vulgaris alcoholic extract that showed zone inhibitions of (38.66, 26.66 , 24, 19.33, 8.66) mm respectively.                                                        

    E. coli was found to be sensitive for di mixed alcoholic extract of (Menthe pulegium and Thumus vulgaris) rcoreded zone inhibition 26.66mm although sensitive from di mixed of (Cuminum cyminum and  Thumus vulgaris) alcoholic extract by zone inhibition 17.33mm while Cuminum cyminum alcoholic extract showed zone inhibition of 9.33mm.

    Bacteria Salmonella entritidis was found only sensitive for di mixed of (Cuminum cyminum and  Thumus vulgaris) alcoholic extract recorded zone inhibition 16.66mm.