The Cardioprotective Effect Of Selenium In Myocardial Ischemia Reperfusion Injury

Abstract

The objective of the current study is to assess the possible cardioprote-ctive effect of selenium in myocardial ischemia reperfusion injury induced by ligation of coronary artery in a male rat model. 24 adult male spraguedawley rats were randomized into 4 equal groups: (1), Sham group, rats underwent the same anesthetic and surgical procedures as the control group except for LAD ligation; (2), Active control group, rats subjected to regional ischemia for 30 min by ligation of LAD coronary artery and reperfusion for 2 hours; (3), Control vehicle group, rats received D.W (vehicle of selenium) via IP route and subjected to ischemia for 30 minutes before ligation of LAD coronary artery & reperfusion for 2 hr; (4), Seleniumtreated group, rats pretreated with selenium5mg/kg via IP injection 30minutes before ligation of  LAD coronary artery & then subjected to reperfusion for 2 hr. In control group, as compared with sham, tissue TNF-α, IL-6, IL-10, caspase-3 and BAX, plasma cTn-T and serum MDA significantly increased (P<0.05), while serum GSH significantly decreased (P<0.05). Histopathologically, control group showed a significant cardiac injury (P<0.05) compared with sham group. Selenium significantly counteracted (P<0.05) the increase of TNF-α, IL-6, caspase-3 and BAX and counteracted the increase in plasma cTn-T and serum MDA. Selenium produces a significant elevation (P<0.05) in cardiac IL-10 and serum GSH with significant reduction in (P<0.05) cardiac injury. In Conclusions, Selenium minimizes myocardial I/R injury in male rats via interfering with inflammatory reactions and apoptosis which were induced by I/R injury .