Role of DPP (Phoenix dactylifera L.) extract on Ameliorating The incidence of hemoglobinoxidation induced by sodium nitrite

Abstract

The effects of ethanolic extract of date palm pollen grains(DPP) was investigated on the ameliorating the  oxidative effect of sodium nitrite on hemoglobin in albino mature female rats , twenty four(24) rats weighing 150- 200 g were grouped into four groups two control and two experimental groups, first group(GI) considered  as negative control received 0.5 ml distilled water and after 45 minutes received another 0.5 ml of  distilled water  while the second control group(GII), (positive control) received 0.5 ml of distilled water then after 45 minutes received 100mg/kg Body weight sodium nitrite. While the two experimental groups were treated as follow, first treated group(GIII) received 100mg/kg Body weight crude ethanolic extract of DPP then after 45 minutes received 100mg/kg Body weight sodium  nitrite. while second(GIV) treated group received 100mg/kg Body weight crude ethanolic extract of DPP  daily for seven days then after 45 minutes from last dose received 100mg/kg B.W. sodium nitrite. Whole blood samples were obtained in heparinized tubes to detect methemoglobin percentage. The results in animals treated with single oral dose (100mg/Kg) of sodium nitrite comprised 45%  met-Hb, 45 minutes after nitrite treatment, while animal^,s group  pretreated with ethanolic extract of DPP in a dose of 100mg/kg for seven consecutive days  given as single oral dose of sodium nitrite after 45 minutes from last dose (before induction of methemoglobinemia) by nitrite administration  revealed an increases in the level of met-Hb percentage  in blood compared to that of control group treated with distilled water before sodium nitrite. However the percent inhibition of met-Hb formation over long term treatment was less than that observed with short term treatment.