The cytotoxic effect of olive oil, nigella sativa and their combination on Hela cancer cell line (in vitro)

Abstract

This study was done to comparison among the cytotoxicity of olive oil, nigella sativa oil and the mixture of olive oil and nigella sativa oil on Hela cancer cell line, the results reveled to the maximum cytotoxic concentration of each olive oil and nigella sativa oil on Hela cancer cell line started from (1 µg/ml – 10000 µg/ml) at 72 hr. incubation periods, which reflect the pattern of cytotoxicity was time depending.

While the result the mixture cytotoxicity shows an increasing in cytotoxicity occurred with increasing in the incubation periods especially at 72hr. at concentration equal to 10000  µg/ml , which give indication about the mixture cytotoxicity was time and concentration depends ,

The cytotoxicity of the mixture was less when comparing with the cytotoxicity of olive oil and nigella sativa oil reflection an antagonism may occurred between the active ingredients of each olive oil and nigella sativa oil causing decreasing in the cytotoxicity of the mixture toward Hela cancer cell line .