Comparative study between the effect of stem cells and Metacam® on induced arthritis in dogs

Abstract

Objective of this project was to study the effect of stem cellson the induced arthritis in the dogs as compared with treatment Metacam^®. Incomplete fruend'sadjuvant was used to induce arthritis. Twenty five dogsaged 12 month were divided into five groups: 1^stgroup (T1) was negative control, while 2^nd group (T2) was induced without any treatment (positive control), 3^rd group (T3) was induction arthritis and treated with 0.2 mg/kg B.W.ofMetacam^®intravenously (I/V)  injection, 4^th group (T4) was induced and treated with 1ml/kg of B.Wof suspension of culture mediainjected (I/V),and 5^thgroup (T5) was treated withstem cellssuspension in a dose of 2.5x10⁶ cells / ml / Kg B.W. (I/V) injection.Typical clinical signs of arthritis appeared on all induction groups.The results of Hb and RBCs showed clear reduction in all induction groups as compared with (T1), the WBCs, Erythrocyte sediment rates (ESR) and C- reactive protein (CRP) in (T2) showed clear elevation as compared with (T1), After 15 and 30 days of treatment the RBCs countand HB level showed significant decline in T2, T3and T4as compared with T5 and T1, theWBCs ,ESR and CRP significantly elevated in the T2andT4as compared with T1. The T3 and T5 showed clear depletion in levels of WBCs, ESR and CRP as compared with T2 after 15 and 30 days of treatment, while the T5 revealed no significant difference as compared with T1 after 30 days of treatment.The histopathologicalchangesin T2 and T4 showed erosion and infiltration of inflammatory cells, T3 showed hypertrophy of synovial lining and mild inflammatory cells in synovium, T5 explained return the bone tissue to normal.In this experiment concluded a successful use of stem cells in treatment of arthritis without side effect as comparison with Metacam^®.