Some toxicological impacts and invitro antibacterial activity of Datura innoxia extract in rats

Abstract

The  study was carried out to investigate some toxicological effects and in-vitro  antibacterial activity of Datura innoxia dried leaves.The yield percentage of Datura innoxia 95% ethyl alcohol leaves extract was 19%. Phytochemicals analysis of both Datura innoxia leaves powder and  alcoholic extract, indicated the presence of alkaloids, phenols, glycosides, tannins, resins, saponins, flavonoids and steroids which are responsible for toxic and therapeutic effects. The acute toxicological effect of Datura innoxia   ethanolic extract in male rats was  determined by estimation the oral median lethal dose (LD₅₀) by up and down method  was 1214 mg/kg. BW.  The in-vitro antibacterial activity of Datura innoxia ethanolic extract  and its minimum inhibitory concentration activity (MIC)       showed that E. coli   was more sensitive to D.I ethanolic extract  than Staph. aureus, the MIC for D.I extract  against E. coli was less than MIC against Staph. aureus which was 5 and 10 mg/ml respectively, also E. coli was more sensitive to ciprofloxacin .