Study comparison analgesic, antipyretic and anti-inflammatory activity of aqueous and alcoholic leaves extract of Lawsonia inermis L. (Henna) with ketoprofen in male albino rats

Abstract

The pain considered is an important signs for many medical condition which is reduced by using analgesic drugs, that act either locally or systemically via central nervous system, that may have many harmful side effects. This study was carried out to evaluate the analgesic, antipyretic and anti-inflammatory effect of both aqueous and alcoholic extract of Lawsonia inermis L. and comparing with one of non-steroidal anti-inflammatory drugs (NSAID) Ketoprofen by using hot plate test, tail immersion method, and study ability of aqueous and alcoholic extract to reduce writhing induced by acetic acid injection. Also, study the antipyretic and anti-inflammatory activity of henna extract in formalin-induced paw edema . Twenty four Wister rats were used which divided randomly into four equal groups, first group served as  control negative treated with distal water, second, control positive, injected with ketoprofen (8 mg/kg B.W.) third group treated with aqueous extract and fourth group treated with methanolic extract (200 mg/kg B.W.) in addition to the ketoprofen. At the end of experiment, the results clarified that alcoholic extract of henna has similar effect of ketoprofen via significant increase (P<0.05) in reaction time to hot plate and tail withdrawal from hot water. Moreover, the results explained significant decrease (P<0.05) in the writhes count( abdominal constriction). As well as, the ability of alcoholic extract to significant (P<0.05 ) reduce body temperature comparing with control group. Likewise, the result revealed that alcoholic extract of Lawsonia inermis has anti-inflammatory activity by significant (P<0.05 ) reduction in edema size induced by formalin injection in paw skin, and Tumor necrosis factor alpha. (TNF-α). Thus, the results of current study illustrated that methanolic extract of Lawsonia inermis has central and peripheral analgesic, antipyretic and anti-inflammatory activity comparing with control group.