Detection the genetic relation between several fungal isolates by using a universal ITS primers

Abstract

The goal of this study is to evaluate the internal transcribed spacer regions (ITS) of the rDNA to detect a phylogenetic relationshep based on PCR technique between four isolates of Trichoderma viride, Chaetumium elatum and tow isolates (pathogen and non-pathogen strains) of Rhizoctonia solani obtained from tomato plant rhizosphere, rice plant rhizosphere and root rotted tomato plants fields of Al-Najaf province, Iraq.

Internal transcribed spacer regions (ITS) of the ribosomal DNA was amplified by polymerase chain reaction (PCR) technique using a set of common universal primers for ITS1, ITS3 and ITS4 regions. Two of three different random primers examined (primers 3 and 4) reflected a specific amplicons within Rhizoctonia solani(1), Chaetumium  elatum , and Trichoderma viride, while the primer 1 did not  give any PCR end product  with all of tested isolates, while Rhizoctonia solani(2) isolates did not revealed any band detected in PCR technique with all primers, also data showing a diferent degree of genetic similarity depanding on PCR data analysis using UVIBand softwear.

Results of this study indicate that ITS set of primers can used to find a genetic relation between fungi.