Comparative study of residues of Levofloxacin and Spiramycin in normal and infected tissues of chicken by HPLC

Abstract

The purpose of this study was to compare the residues of Levofloxacin and Spiramycin in chicken plasma, kidney and breast muscle tissues  by HPLC assay in( normal and infected tissues with E coli)at ( 0,3,5,8) day. After the last therapeutic dose. Sixty broiler chicken with 30 days old of mixed sexes were used in the study. They were divided in to 5 groups: control group (non- infected – non treated),normal birds   received levofloxacin at 10 mg per kg body weight orally for five days, birds  received Spiramycin (15mg/ kg) body weight orally for five days, Birds were challenged with E. coli and treated orally with Levofloxacin , Birds were challenged with E. coli and treated orally with Spiramycin . and placed in the animal house at Faculty of Veterinary Medicine, Kufa University .The result of  residue concentration of two drugs were gradually decreased in all tissue samples from day zero to day eight after the last dose .Concentration in kidney more than muscle  tissue for two drugs levofloxacin and Spiramycin  and concentration in infected tissue more than normal tissue(µg/gm) ,levofloxacin concentration were( 0.069±0.002,0.106±0.004 )( µg/gm). in normal and infection kidney respectively  in day 5 after the last therapeutic dose 10mg/kg /B.W orally for 5 days  ,which less than maximum residue limit ( MRL), (0.2µg/gm).Will  Spiramycin concentration in infected and normal kidney tissue in day five after the last dose with therapeutic dose 60000 IU/kg/B.W were0.720 ±0.06 ,0.349±0.03 respectivly,more thanMRL(0.30µg/gm),therefore the levofloxacin,less residue in tissues  and  low withdrawal time. Thane Spiramycin  .Aim of the study:This work study the safety of two drug by estimation residue in plasma and tissue andwithdrawal timefor  normal case and infected animals with avian E coli.Following oral administration  at the therapeutic  dose for five days in  the chicken.