Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in Al-Muthanna Province- Iraq

Abstract

klebsiella is the most common pathogen responsible for acquired infections in the hospitals and because of the extensive ability to produce β-lactamases made this bacteria resistance to many antimicrobial agents. This study was done to determine Klebsiella pneumoniae isolates containing both blaFOX and blaCIT by multiplex polymerase chain reaction . Out of fifty one Klebsiella isolates , 31 (60.8%) K. pneumoniae isolates were resistant to cefoxitin by disk diffusion method . The production of molecular class C β-lactamase tested through modified three dimension method in all cefox resistance isolates and observed 14 (45.2%) isolates were producing for AmpC β-lactamase . The cefoxitin resistance isolates further screened by AmpC disk method and the result was 11 (35.5%)isolates were producing for AmpC β-lactamase . Multiplex PCR technique showed 8/31 (25.8%) K. pneumoniae isolates possess AmpC β- lactamase gene as well as this technique also revealed that FOX gene was exists in (37.5%) and CIT gene (62.5%)of the isolates .