Synthesis, quantification and characterization of 1,3,4-thiadiazole derivatives of ampicillin by HPLC

Abstract

In this study synthesized new Ampicillin derivative and diagnosed using FTIR and HNMR Technique. Also, in this study, an accurate, rapid, and sensitive chromatographic method was developed to separate the prepared derivative from the standard drug, and then this method was applied to pharmaceutical preparations and This method was linear, accurate, and specific. and The optimal conditions used in this method were is Mobile phase (%80 H2O containing 0.1% acetic acid + %20 Acetonitrile v/v), Nucludar C18-DB column, particle size 3 μm (50 x 4.6 mm I.D), flow rate 2 mL/min, pH = 4.5. The injection volume was 20 L, the wavelength was 210 nm, the run time was 6.00 minutes, and the linearity ranged from 0.1 to 10 μg/ml. Ampicillin has a retention time of 4.105 minutes. R2 was found to be 0.9988 for the standard medication, and the LOD and LOQ for Amp were determined to be (0.01 μg/ml and 0.033 μg/ml) respectively.