New spectrophotometric determination sulfamethoxazole drug via various analytical methods in pharmaceutical formulation

Abstract

For the determination of sulfamethaxazole (SMZ) in pure and pharmaceutical formulations, new simple sensitive spectrophotometric methods have been devised. The first technique involved forming an ion pair complex between the basic nitrogen of the SMZ medication and Eriochrome black (EBT) in an acidic buffer solution, and extracting the created complex with chloroform to get a persistent reddish-orange colored dye with a maximum wavelength of 490 nm. Beer's law was followed, and 0.9998 was the correlation coefficient., 0.471×104L. mol^-1.cm^-1was the molar absorptivity, and the detection limit was 0.177 g/mL. Dispersive liquid-liquid extraction (DLLE) is a second method for estimating a trace amount in an aqueous solution product using an ion pair and a UV-visible spectrophotometer with a maximum wavelength of 490 nm. The concentration range was 0.5-6 g/mL, with a correlation coefficient of 0.9995, 0.889×10⁵ L.mol^-1.cm^-1, a molar absorptivity of ,the detection limit of 0.013 g/L, and a pre-concentration factor of 25 and an enrichment factor of 18.20. Sulfamethaxazole in pharmaceutical formulations was successfully determined using the provided methodology