Evaluation of liver enzymes activity and some trace elements in sickle cell anemia patients in Basrah Governorate – Iraq.

Abstract

 Sickle cell anemia is one of genetic diseases caused by hemoglobin biochemical disorder in especially in polypeptidic chain of this protein. The study was conducted from October 2019 to December 2020 and included two sets of samples Patient and Healthy .The current study was curried out to evaluate some enzymes and trace elements in patients with sickle cell anemia. The levels of copper (Cu) were estimated spectrophotometrically by reaction of copper with 4 – (3,5 – dibromo – 2- pyridylazo) – N – ethyl sulphopropylaniline to produce a chelating complex.The determination was carried out at wavelength equal to 520 nm. Finally, absorbances were recorded and they are proportional to concentration of copper in the sample . Also, the levels of zinc in serum of blood belonging to sickle cell anemia (SCA) were investigated and estimated by reaction of zinc with chromogen existing in the characteristic kit to produce coloured complex. The absorbances were recorded spectrophotometrically which are proportional to concentration of zinc in blood sample . The levels of biochemical variables were estimated using ARCHITECT c4000 systems provided by ABBOTT Company, which are designed with advanced technology, accuracy in clinical results and ease of handling. The device uses a sample size at a rate of 6µL in all measurements of chemical variables.The total values of the current results were made and expressed by calculation of the mean ± standard deviation. Analysis of all results data were  carried out by using a statistical procedure according to analysis of variance univariate . Statistical programmer of social science  (SPSS ,v.23 ) was depended for calculation of significant differences for the different groups of patients infected by SCA disease and healthy persons . The correlation regression coefficient (r) was used and depended to distinguish between the means of groups belonging to all patients and healthy individuals ( control group ) .The p-value was agreed for less than 0.05 to the limit of lowest significance . A highly significant difference was recorded belong to levels of GPT, ALP and GOT in patients compared with healthy individuals (control group) according to age and sex factor.