Application of Cloud Point Extraction of Amoxicillin Determination using Liquid Ion Exchange method Compared with HPLC in Pharmaceuticals

Abstract

A direct and compassionate approach has been examined to quantify Amoxicillin (AMX) in pharmaceuticals. This method involves a liquid ion exchange process that relies on forming an ion-pair association complex between AMX and Co (II) as a chloroanion complex by the presence of hydrochloric acid and the non-ionic surfactant Triton X-100. The highest wavelength recorded was 357nm. An analysis was undertaken on the impact of the optimal conditions on the separation process, specifically at a temperature of 95°C. After a heating duration of 15 min., the Co(II) concentration was determined to be 100µg/mL, with an optimal TritonX-100 surfactant volume of 0.5mL. Furthermore, an investigation was conducted to analyze the impact of various organic solvents with differing dielectric constants on the dissolution of the Cloud Point Layer. This study aimed to elucidate the most likely structure of the extracted complex was 1:1 [AMX]+; HCoCl4-. The linear calibration curve spanned the concentration range of 5-1000 μg/mL, exhibiting a limit of detection (LOD) of 4.790 μg/mL and a limit of quantification (LOQ) of 14.515 µg/mL. The analysis of Amoxicillin in pharmaceutical samples was conducted using the CPE-ILE method compared to HPLC, aiming to enhance the precision of the CPE-ILE method for determining Amoxicillin.