Cyclic imides , azo dyes: Synthesis, Identification and biological activity.

Abstract

The research included the preparation of cyclic imide compounds (M1-M4) from the reaction of the amino compound 4,4-methylene diniline with various cyclic anhydrides (malic, succinic, Phthalic, and 1,8-naphthalic) in one step and by the microwave method. Azo dye compounds (M5-M9) were prepared in two steps. The first was preparing the diazonium salt of the amino compound with sodium nitrite and HCl. The second step was the reaction of the diazonium salt with the coupling materials (2-naphthol, vanillin, salicylic aldehyde, salicylic acid, 8-hydroxyquinoline) . The prepared compounds were characterized by several spectroscopic methods, including FT-IR, 1H, 13C-NMR, and mass spectrometry. The prepared compounds (M1-M9) were tested against two types of bacteria: Staphylococcus aureus, which is gram-positive and Escherichia Coli, which is gram-negative.

The results showed that some compounds have high effectiveness against E-Coli bacteria, such as compounds M3 and M6, and compounds M1, M2, M5, M8, and M9 showed high effectiveness against Staphylococcus aureus bacteria compared to the standard antibiotic, gentamicin sulfate, at a concentration of 6.12 mg/ml.

The compounds (M3, M4, M6 and M9) also showed excellent effectiveness against the fungus Candida albicans compared to the standard antibiotic Nystatin.Compound M5 has shown to be a good azo dye in the dyeing process (cotton, wool, brocade and wood.