An Enzymological and hormonal comparative Study Between Obese And Normal Weight In Iraqi Females With PCOS

Abstract

Background Polycystic Ovary Syndrome (PCOS) is defined as a series of interconnected reproductive disorders, including as irregular gonadotropin secretion, elevated testosterone levels, persistent anovulation, and polycystic ovarian structure. It is often linked to insulin resistance and obesity. These reproductive and metabolic disorders result in significant morbidities throughout life, including anovulatory infertility and type 2 diabetes (T2D)".Objectives enzymes activities (aromatase and 3β-Hydroxysteroid Dehydrogenase ) and hormones levels (estrogen and DHEA-s) disturbances between obese and non obese ( normal weight ) polycystic women and highlight the outcomes according to the body weight Methods The study design is the case-control, where (60) Blood samples have been taken from polycystic  individuals at the Maternity and Children's Hospital. divided into  ( G1) obese women with PCOS / 30 samples, while group 2  (G2) consists of 30 samples of normal weight PCOS women,. The activities of Aromatase, the 3β-Hydroxysteroid Dehydrogenase enzymes, and others hormones  were determined and the difference were analyzed by analysis of results by one way (ANOVA).Results A non significant decrease of Aromatase activity between obese (G1) and non-obese patients (G2) (3.56±2.40), (5.25±4.88) respectively. Also, a significant decrease in the levels of 3β-Hydroxysteroid Dehydrogenase was reported between G1 and G2 (3.39±1.09),( 4.66±1.43) at respectively at P-value less than 0.05 was reported.,And There are significant decrease of estrogen and DHEA-s hormones for the obese (G1) and non-obese patients (G2) (82.20±25.10), (169.24±38.25) , (347.13±90.56), (243.06±83.71)  at respectively at P-value less than 0.05 was reported. Conclusion The present study is the first in Iraq  reporting that obese women with PCOS have reduced amounts of the enzyme Aromatase, 3β-Hydroxysteroid Dehydrogenase, and reduced level of estrogen while DHEA-s  level was increased in the same patients compared with PCOS patients with normal weight. The clinical biochemistry lab uses the disruptions in hormones and enzymes As a diagnostic tool for this disease by highlighting the obesity.