A Novel Approach for the Rapid Detection of Sulfanilamide in Pure and Formulated Samples Using Cloud Point Extraction

Abstract

To determination of sulfanilamide (SNA) as a pure and pharmaceutical forms by easy, fast, and sensitive method. A liquid ion association complex of SNA formation by NiCl₄^-2 in the presence of nonionic surfactants TritonX-114 and mixture of (TritonX-114+Tween80) to form cloud point layer. The highest absorption at 277nm with both surfactants. The optimum conditions effect on the separation method was studied: concentration of HCl (0.8M), temperature (75℃), heating time (15min), the concentration of Ni (II) was 100μg.mL^-1, and (0.5mL) of both surfactants. The calibration curve was linear over the concentration range of 10-100μg.mL^-1with LOD of (0.984 μg.mL^-1) and LOQ of (2.98 μg.mL^-1) with TritonX-114 and LOD of (0.9936μg.mL^-1) and LOQ of (3.011μg.mL^-1) with mixture (TritonX-114+Tween80). SNA spectrophotometric determination was carried out by CPE and compared with HPLC. The results showed no significant differences between the two methods.