Cloud Point Extraction and Spectrophotometric Determination Zinc II Ion Complex in Real Samples Using BTAD Reagent

Abstract

          New azo reagent 2-[Benz Thiazol azo]-1 –Dopamine , The reagent was Prepared through a coupled reaction and characterized using spectroscopic methods, including (FTIR) and (UV. –Vis.) is spectroscopy , as well as proton nuclear Magnetic resonance ( HNMR).to verify its chemical identity .This reagent was used to estimate Zinc( II ) ion through the formation of  a doubly charged complex, Which was extracted and identified using  cloud point extraction(CPE) technique , Optimization conditions and factors affecting is study that effected on extraction Efficiency E%. The measured of spectrophotometrically at a wavelength  of at λ _max =515 nm for Zn⁺² complex .  The  conditions for  complex formation and extraction Using  (CPE)technique were studied and optimized , and the optimum pH is found =9 , critical volume the Triton X-100 concentration is at 0.5 mL, heating time is 85 C˚. Thermodynamic study of parameters to CPE are also calculated ,and their values Were as follows  ΔH =0. 243874 KJ ,ΔG=-0. 181914134 KJ and  ΔS =50.8821 J  Indicating that the reaction is endothermic (LOD=6.4486x10^-6µg mL^-1) and LOQ= 6.4486x10^-5µg mL^-1 , with( Molar absorptivity Ɛ=286.88L.mol^-1,cm^-1. The  Stoichiometry of the ]Zn⁺²(BTAD)[ showed that the ratio of M:L obtained are (1:1). at  optimum  conditions , the analysis 10 mL of sample yielded  pre concentration Factor and enrichment factor   of (200 and65.92366) respectively,  while the Sandal's sensitivity(4.1x10^-6 µg cm^-2).Standard calibration curve was  linearity  at Range between  0.05-1ppm, with a correlation coefficient of 0.9933. The RSD% of Repeated measurements by a concentration  of determinations at (90) µg 10mL⁻¹ is  74.11%this developed method was applied to estimate Zinc concentration in Different samples and compered whit  FAAS for samples and use t-test to compered The results accept Nell hypothesis and this method can be depend at confidence Limit 95%.