Synthesis and Identification of Some New Mono- and Bis-Tetrazole Derivatives with an Evaluation of the Free Radical Scavenging Activity of a Selected Compound

Abstract

The research involved the preparation of mono- and bis-tetrazole ring derivatives from the 4,4-thio-dianiline compound as a substrate. These derivatives were prepared using two methods. The first method was a multicomponent reaction, where 4,4-thiodianiline reacted with sodium azide and triethyl orthoformate in glacial acetic acid as a solvent with reflux. The second method involved converting 4,4- 4,4-thiodianiline to an organic azide derivative by reacting diazanium salt with inorganic azide, followed by reacting the organic azide with phenyl isothiocyanate. In addition, a chloroacetamide derivative containing a tetrazole ring was prepared using an SN₂ reaction. The characterization of these compounds was conducted by measuring their melting points and using FT-IR, ¹H-NMR, and ¹³C-NMR spectroscopic techniques. Compound C3, which contains a tetrazole ring group with a free aromatic primary amine group, was selected for investigation as an antioxidant compared to Ascorbic acid.