Protective effect of captopril against methotrexate- induced nephrotoxicity in mice

Abstract

The present study was designed to investigate some histopathological changes produced by methotrexate on kidney in mice and extended to examine whether there using captopril have ability to reduce this toxicity.

Forty mice(20 male and 20 femel) were divided in to 4 groups .  The first group received 10mg/kg B.W. of methotrexate I/M twice weekly. The second  group  received  60mg/kg  B.W. of captopril and then received methotrexate. While the third group received captopril alone,While fourth group (control group) received normal saline and the study was continued for (30 days).

    Animals which  were  treated  with  methotrexate  only  along  the  period of experiment  showed  sever pathological changes,   characterized by severe   tubular necrosis ,atrophy of glomeruli and dilatation of tubules.Where as oral administration of captopril significantly(p<0.01) reduced these changes .The protective activity of captopril has been resumed via diminishing the renal histopathological changes which associated with methotrexate Toxicity.while there were insignificant differentiated (P<0.01) between the control group and the group recieve of captopril alone.