Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria

Abstract

 Peganum harmala L. (Zygophyllaceae) used in traditional medicine of Iraq and itis one of the most famous medicinal plants. The most bacteria pathogen is resistance for synthesis antibiotic drugs resulting from abuse uses of the patient or produce resistance strains for microorganism therefor,  the aim of this search is the effect antibacterial of seed alcoholic extract of peganum harmala  on some Gram negative bacteria pathogen. Methods: The broth serial  dilution assay method determined the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of seed methanolic extract of Peganum harmala   were tested for antibacterial effect against bacterial isolates (salmonlla enteric , klebsiela pneumonia multidrugresitant E. Coli and highly resist E.coli), used phytochemical analysis of seed mathanolic extrac of peganumharmala. Result: showed good antibacterial activity against strain  Salmonella enteric and Klebsella pneumonia, were MBC at concentration (25 mg/ml) and no effect of low concentration of MIC. The MBC value against strain multidrugresistant Escherichia coli, was at concentration (3.125 mg/ml) and MIC at (25 and 12.5 mg/ml), while MBC of highly resist Escherichia coli was in (12.5mg/ml) and MIC value at 50 mg/ml compared with standard antibiotics synthesis. Phytochemical analysis of component seed methanolic extract of peganumharmala showed presence of flavonoids, alkaloids, saponins, tannins, glycosides, terpenoids and steroids.