Efficacy of Silver Nanoparticles, Antimicrobial peptides and Blue Light Combination on Healing Infected Wound by Methicillin Resistance Staphylococcus aureus in Rats

Abstract

This study was carried out to evaluate the effectiveness of the triple combination of biologically synthesized silver nanoparticles, anti-microbial peptides and visible blue light against pathogenic clinical methicillin resistance Staphylococcus aureus (MRSA) in vivo after inducing full thickness skin injury in the initially induced diabetic rats. This experiment of in vivo study was for evaluation the role of the combinations in promoting wound healing after wound inducing infection using pathogenic MRSA (0.25ml from 1.5 ×10⁸ cfu/ml) in diabetic rats. 108 diabetic rats (aged 8-10 weeks weighing 200-250g) were divided equally into nine groups, (treatment started after the appearance of infection signs). Group A: Negative control group, group B: Positive control group, Group C: infected treated with AgNPs 8µg/ml, group D: infected treated with Gramicidin 16µg/ml, group E: infected treated with blue light for 1 hour, group F: infected treated with combination of AgNPs 0.25µg/ml with blue light for 1 hour, group G: infected treated with combination of Gramicidin 8 µg/ml and blue light for 1 hour, group H: infected treated with combination of AgNPs and Gramicidin 0.25µg/ml, 0.5 µg/ml respectively, group I : infected treated with triple combination of AgNPs ,Gramicidin 0.25µg/ml, 0.5µg/ml respectively and blue light for 1 hour. All groups treated topically daily for three days. Wound healing was assessed by measuring wound contraction at 7, 14 and 21 days post wound induction. Viable bacterial count at 21 day post wound induction. Results were according to data analysis, healing rate of treated rat with the triple combination was significantly (p < 0.001) faster (within 7 days) than treated rat with other double combination and each agent alone. All symptoms returned to near normal values at the end of treatment. In conclusion the topical therapy of the triple combination of AgNPs, visible blue light and antimicrobial peptides promote wound healing process in diabetic infected wound in a short period of time. A new approach can be used to combat serious infections caused by    MRSA by combining AgNPs, blue light, and antibiotics.