Direct detection of "methicillin resistant Staphylococcus aureus" from buffalo raw milk in Al-Qadissiya province using Polymerase Chain Reaction assay

Abstract

The shedding of "methicillin resistant Staphylococcus aureus" in raw buffalo milk causes a potential risk if consumed without maintaining sufficient hygienic criteria due to numerous clinical implications to human infection. The present study was used the Polymerase chain reaction technique as highly specific molecular procedures for direct detection of "methicillin resistant Staphylococcus aureus" (MRSA) of buffalo raw milk samples that compiled from animals owners of different areas in the province of Diwaniyah during the period from July 2016 to December 2016. PCR technique was dependent on used specific primers that amplification of mecA gene in Staphylococcus aureus. This primer was designed in this study by using NCBI-Genk data base (KM505043.1) and primer 3 plus for primers design. The PCR results were shown that buffalo infected with (MRSA) at 8 positive samples at percent (16%) out of 50 milk samples.
The purpose of this research was to establish a rapid and specific PCR Technique for the diagnosis of "methicillin resistant Staphylococcus aureus" in buffalo raw milk that will be used as alternative to the currently available convention detection methods and makes it possible to identify the foods at risk for MRSA contamination to public health.