The Role of Klebsiella Pneumonia for Effect on Pneumonia in The Sheep

Abstract

The study investigated the pathology changes associated with klebsiella pneumonia in lungs of sheep slaughtered. The objective of this study was focus on molecular detection and genotyping were done by polymerase chain reaction and gel-agarose electrophoresis. The molecular detection of capsular polysaccharide gene (cps) was investigated using PCR specific primers. PCR was performed with the primer that target the 16S rRNA. PCR was performed with the primer that target the 16S rRNA . Genotyping of these isolates were also carried out by using K1, specific PCR primers. When using the primer specific for the capsule cluster gene magA ( K1serotype). A total of 700 lungs of slaughtered sheep were examined individually and out of which 220/700 (31.4%) affected lungs were collected for histopathology and bacterial isolation, during the period from October 2015 to February 2016. 30 / 220 isolates were identified as klebsiella pneumonia (13.6%) ,according to characterization of morphology and biochemical for this microorganism, VITEK 2 system and confirmed by using the primer that target the 16SrRNA. Grossly, the lung lesions were categorized into: (1) solidation of lung (40%) ,(2) hepatization in lung 30% ,(3) abscesses in lung (10%) , (4) edema and fibrosis (10%) and congestion and Edema (10%). In histopathology examination, lung lesions were categorized into: (1) Suppurative bronchopneumonia (36.6 %) ,(2) interstitial pneumonia (30%) ,(3) bronchointerstitial pneumonia (13.3%) , (4)pleuritis with pulmonary edema (10%) and (5) pulmonary abscesses (10%).Special stains were used to verify the lesions. Collagenous fibers were stained red color with Van Gieson’s stain, polysaccharide were stained pink color with PAS stain. PCR technique showed that 19 isolates were positive to K1 serotype and 11 isolates were negative for K1. These results suggest that magA genotype might be a useful marker to identify K1 serotypes of K. pneumoniae.