Role of Testosterone in Serum and Follicular Fluid in Sub Fertile Women Undergo Intracytoplasmic Sperm Injection

Abstract

Background: Testosterone is the most common and regarded as the main hormone in the testicles among androgen (Tremblay, J. J. (2015) Testosterone synthesis is initiated by luteinizing hormone and cAMP signaling, promoting the transport of cholesterol to the inner mitochondrial membrane with the involvement of the steroidogenic acute regulatory protein (StAR). Cholesterol is then converted into pregnenolone, which further transforms into progesterone and is eventually metabolized into testosterone. This steroidogenesis pathway is similar in both thecae cells and interstitial cells of the ovaries (Cipolla-Neto, J., et al., 2022). Low-quality oocytes with a propensity for lower cleavage rates following fertilization have been associated with elevated androgen levels, more especially testosterone, in follicular fluid (FF) (Costa, L. O. B., et al., 2004). When FF contained immature oocytes as opposed to mature oocytes, testosterone levels were found to be noticeably higher. Pregnancy-associated follicles were found to have a higher estrogen-to-testosterone (E2/T) ratio reported. These results raise the possibility that an early follicular atresia linked to a low estrogen/androgen ratio in FF may reduce the viability of the oocytes and reduce the likelihood of fertilization and pregnancy purpose: to detect the follicular fluid and blood concentration of the testosterone hormone on subfertility in women undergoing Intracytoplasmic Sperm Injection (ICSI) for fertility treatment. Methods: This case-control study was carried out on eighty women, from these, forty females were sub-fertile patients defined as the cases, other forty females as a control included fertile females came to fertility center due to males factor of subfertility. A detailed medical history and physical examination were recorded. At the same day of ovum pickup blood and follicular fluid were collected, for hormonal analysis of testosterone. Results: Show significantly higher levels of serum and follicular testosterone in sub-fertile women (cases) than control group (p < 0.05), and significant positive correlations between serum and follicular fluid. Additional materials: notes the number of references, tables, graphs, exhibits, test instruments, appendixes, or other supplemental materials in the paper. Also, the abstract must be written in a single paragraph in English, max 250 words.  Conclusion: Testosterone hormone level in blood and follicular fluid were significantly higher in cases group than control and there is a positive correlations between serum and follicular fluid of testosterone.