Effects of Pirfenidone on Renal Ischemia-Reperfusion Injury in a Mice Model: Reno-protective Effect

Abstract

This study look to the nephroprotective properties of pirfenidone in adult male mouse model with renal ischemia-reperfusion injury (IRI). Adult mice (12-15 weeks age and weigh 25-35 g) were separated at random to four groups: sham, ischemia, vehicle (DMSO), and pirfenidone (300 mg/kg orally, 30 minutes preceding to ischemia). Renal IRI produced by 30 minutes of bilateral ischemia and 2 hours of reperfusion. In order to assess renal function and oxidative stress, serum urea, creatinine and F2-isoprostane was checked. TNF-α as well as NF-κB levels in renal tissue also was checked for reactions related to inflammation. Histopathological grades evaluated structural kidney injury. When compared to sham group, the ischemia and vehicle groups revealed significant higher levels of renal function, oxidative stress, and inflammatory markers. Pirfenidone lowered all biochemical indicators dramatically, while improving histological damage scores, resulting in near-normal architecture. The study found that pirfenidone provide significant nephroprotection in renal IRI by acting as anti-inflammatory and antioxidant.