Evaluation of Advanced Glycation End Products and other Biochemical Parameters in Patients with Leukemia

Abstract

This study's objective is to assess the Advanced glycation end products (AGEs), oxidative stress and antioxidant parameters in sera of leukemia patients and control group. In the present study we measured the concentration of serum levels of carboxymethyl-lysine (CML), pyrraline, Nitric Oxide (NO), Malondialdehyde (MDA), superoxide dismutase (SOD), catalase and glutathione (GSH) in leukemia patients and control group. This study was performed on 80 individuals, 50 children, men and women with leukemia patients (20 females and 30 males) and 30 healthy individuals (children, women and men, 12 females and 18 males) as control group who had no leukemia. The results show that the median and mean serum level of each CML, MDA and GSH are significantly, but CAT and SOD are insignificantly higher in leukemia patients than in control group (CML= 479.3 (411.1, 545.4) ng/ml (p value <0.0001), (MDA= 175.7 (160.8, 191.8) ng/ml (p value= 0.0357), (GSH= 5.772 ± 0.3615) μg/ ml (p value= 0.0194), (CAT= 6.857 (6.281, 7.425) ng/ml (p value= 0.8193) and (SOD= 1.111 (0.8918, 1.478) ng/ml (p value= 0.0891). The median serum level of Pyrraline and NO are insignificantly lower in leukemia patients compared to control group (Pyrraline= 156.9 (137.3, 179.0) pg/ml (p value = 0.8995) and (NO= 119.3 (96.66,192.6) μmol/L (p value= 0.2289). The correlation analysis demonstrates that there are significantly positive correlations between serum CML and MDA (r= 0.3487) (p value= 0.0152) and between serum pyrraline with MDA (r= 0.3838) (p value = 0.0093) and GSH (r= 0.3371) (p value= 0.0236).