Acute inflammatory responses and histological changes for Stachybotrys chartarum fungus in the lungs and nose for infant white mice

Abstract

     Stachybotrys chartarum in one of fungi that cause respiratory system infection to human and animals therefore this study it done to evaluation histological changes in some  respiratory organs such as nose and lung induced by this fungus suspension. Detection of Stachybotrys chartarum fungus confirmed  by cultured  samples which collected from baths floor on potato dextrose agar  in room temperature for seven days. Then the suspension of positive samples of this fungus diluted by phosphate buffer saline and counted the spores by using hemocytometer before used experimentally in lab animals (Balb/C mice) to study histological changes in respiratory system . Twelve mice used in this study divided into two groups. first group consist of six mice  induced with   0.5 ml from one positive sample of fungus suspension contain 2×10⁴ spore / mm³(for month : one dose daily) according to count of hemocytometer to evaluate some histopathological changes  in nose and lung .Second group induced with 0.5 ml from phosphate buffer saline only. The results revealed that the nose of mice injected with 0.5 ml contain 2×10⁴ spore / mm³ from Stachybotrys chartarum  fungus suspension cause focal superficial necrosis of the respiratory  epithelial cells with acute inflammatory  cells  and infiltration of the mucosa .while the lung of group mice treated with same  concentration  of fungus  has interstitial pneumonia  with thickening  of inter alveolar  space  due to infiltration  and chronic inflammatory cells especially mononuclear cell.