Detecting Phenotypic and Genotypic of the Antibiotic Resistant Salmonella enterica Serotype Paratyphi Isolated from Blood Samples in Najaf Province /Iraq

Authors

  • Fadhil H.N. Al-Muhannak Department of Medical Microbiology, Faculty of Medicine, University of Kufa , Iraq.
  • Hashim Ali Abdualmeer Al-sherees Department of Medical Microbiology, Faculty of Medicine, University of Kufa
  • Ibrahim Abed Ali Abdul Sada Najaf Health Directorate, Najaf, Iraq
  • Thikra Abdullah Mahmood Department of Medical Microbiology, faculty of Medicine, University of Kufa, Iraq
  • Rasha Fadhel Obaid Al-Mustaqpal University college, Babylon, Iraq

DOI:

https://doi.org/10.36330/kmj.v18i2.10292

Keywords:

aac(6')-lb genes, blaCTX-M, blaOXA, blaSHV, blaTEM, ESBLs, S. Paratyphi

Abstract

Background & Objectives: Salmonella Paratyphi is a leading cause of human paratyphoid fever in developing countries, causing deaths in humans worldwide. There are several paths for catching paratyphoid fever, but the close contact between patients and healthy humans is so far the most frequent cause of human infections. No study is found about the antibiotic resistance (phenotypic and genetic) of S. Paratyphi isolated from patients in Al-Najaf Province/Iraq, to the researcher’s best knowledge. Hence, this study aimed to determine the prevalence of S. Paratyphi isolates from blood specimens and the antibiotic resistance determinants of them, as well as the genetic relationship among isolates.

Methods and Results: Blood specimens from 1743 patients with suspected enteric fever were cultured for the identification of Salmonella enterica during the period from first April to the end of October 2017. 107 (6.14%) S. enterica isolates were recovered and only two (1.87%) of isolates were S. enterica serovar Paratyphi B. These two isolates (ST39 and ST89) were tested against 23 antibiotics using the disc diffusion method on Muller-Hinton agar and the genotypic antibiotic resistance determinants by PCR. ST39 isolate was sensitive to all antibiotics while ST89 isolate was resistant to only cefepime, piperacillin and tobramycin. ST39 isolate did not carry integrons (1 or 2) and any resistance determinants, while ST89 isolate carried integron class1 as well as blaTEM, blaCTX-M and aac(6')-lb genes.

Conclusions: The findings of S. Paratyphi isolates with integron 1 and resistant antibiotic genes indicating public health risks.

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Published

15-12-2022

How to Cite

Al-Muhannak, F. H., Ali Abdualmeer Al-sherees, H., Abdul Sada, I. A. A., Mahmood, T. A., & Obaid, R. F. (2022). Detecting Phenotypic and Genotypic of the Antibiotic Resistant Salmonella enterica Serotype Paratyphi Isolated from Blood Samples in Najaf Province /Iraq. Kufa Medical Journal, 18(2), 98–106. https://doi.org/10.36330/kmj.v18i2.10292

Issue

Vol. 18 No. 2 (2022): Kufa Medical Journal - December - 2022

Section

Original Articles

License

Copyright (c) 2022 Fadhil H.N. Al-Muhannak, Hashim Ali Abdualmeer Al-sherees, Ibrahim Abed Ali Abdul Sada, Thikra Abdullah Mahmood, Rasha Fadhel Obaid

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