Imaging and Diagnosis of hMPV , RSV and Measles Viruses by Fluorescence Microscope

Abstract

The use of fluorescence microscopy for imaging biomedical specimens has been expanded into all fields of diagnosis for different kinds of viruses , three viruses were imaged and diagnosed by the fluorescence microscope at Al-Sadr educational hospital at Kufa city . The human metapneumo viruse hMPV , the respiratory syncytial viruse RSV and the measles chicken emberyo fibroblast viruse have been imaged and diagnosed by the fluorescence microscope in Al-SADR Eduacational hospital at Kufa city . Three types of dyes were added to the specimen solutions separately ,Rhodamine B , Fluorescene and Evans blue in order to obtain high resolution and contrast images. The absorption and fluorination spectra of these dyes were measured by EUV-visible spectrophotometer and fluorospectrophotometer respectively .