IMPROVEMENT OF ELECTRON MICROSCOPE VIRUS IMAGES THROUGH SEGMENTATION AND CONTRAST ENHANCEMENT
DOI:
https://doi.org/10.30572/2018/KJE/160437Keywords:
Microscope Virus images, Image enhancement, Dark Channel Prior, Adopted Histogram EqualizationAbstract
High-resolution imaging techniques are essential for accurately studying viruses and their effects. Due to their small size, viruses often require sophisticated imaging tools for successful detection and analysis. Microscopy techniques, such as electron microscopy or fluorescence microscopy, are commonly used to capture images of viruses. However, these images can sometimes lack contrast and detail, making it challenging to identify important structural features. This paper introduces a new method to enhance the gray-scale images of 36 electron microscope virus images by increasing contrast and brightness using dark channel prior and adapted histogram equalization used Otsu's segmentation. To determine the efficiency of the proposed method in improving microscopic images, it was compared with several other methods. The quality measurements demonstrated significant success, with entropy (EN) at 7.800, average gradient (AG) at 16.996, mean standard deviation (MSTD) at 44.321, and contrast enhancement measurement (CEM) at 0.8752. The results indicate the algorithm's effectiveness in preserving image clarity and enhancing its features in a superior manner
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Abd-Alameer, S.A., Daway, H.G. and Rashid, H.G. (2020) ‘Quality of medical microscope Image at different lighting condition’, in IOP Conference Series: Materials Science and Engineering. Available at: https://doi.org/10.1088/1757-899X/871/1/012072.
Alaa, R., Hussein, E.A. and Al-libawy, H. (2024) ‘OBJECT DETECTION ALGORITHMS IMPLEMENTATION ON EMBEDDED DEVICES: CHALLENGES AND SUGGESTED SOLUTIONS.’, Kufa Journal of Engineering, 15(3).
Ameer, Z.S.A.-A., Daway, H.G. and Kareem, H.H. (2019) ‘Enhancement underwater image using histogram equalization based on color restoration’, Journal of Engineering and Applied Sciences, 14(2). Available at: https://doi.org/10.3923/jeasci.2019.641.647.
C, E. (2009) Digital image processing. Pearson education india.
DAWAY, E.G., ABDULAMEER, F.S. and DAWAY, H.G. (2022) ‘X-RAY IMAGE ENHANCEMENT USING RETINEX ALGORITHM BASED ON COLOR RESTORATION’, Journal of Engineering Science and Technology, 17(2), pp. 1276–1286.
Daway, H.G., Al-Alawy, I.T. and Hassan, S.F. (2019) ‘Reconstruction the illumination pattern of the optical microscope to improve image fidelity obtained with the CR-39 detector’, in AIP Conference Proceedings. Available at: https://doi.org/10.1063/1.5123076.
Dos Santos, F.L.C. et al. (2015) ‘Computer vision for virus image classification’, Biosystems Engineering, 138, pp. 11–22.
For, N.P.E.A. et al. (2020) ‘Colour image enhancement by fuzzy logic based on sigmoid membership function’, International Journal of Intelligent Engineering and Systems, 13(5), pp. 238–246.
Gergerich, R.C. and Dolja, V. V (2006) ‘Introduction to plant viruses, the invisible foe’, The plant health instructor, 478.
Habeeb, R.M. (2020) ‘Video Images Enhanced by using Sigmoid-Logarithm Transform’, in IOP Conference Series: Materials Science and Engineering. IOP Publishing, p. 12062.
He, K., Sun, J. and Tang, X. (2010) ‘Single image haze removal using dark channel prior’, IEEE transactions on pattern analysis and machine intelligence, 33(12), pp. 2341–2353.
Kadhim, A.M. and Daway, H.G. (2023) ‘Enhancement of Microscopy Images by Using a Hybrid Technique Based on Adaptive Histogram Equalisation and Fuzzy Logic.’, International Journal of Intelligent Engineering & Systems, 16(1).
Kohno, T. et al. (1998) ‘Contrast-enhancement for the image of human immunodeficiency virus from ultrathin section by immuno electron microscopy’, Journal of virological methods, 72(2), pp. 137–143.
Matuszewski, D.J. and Sintorn, I.-M. (2019) ‘Reducing the U-Net size for practical scenarios: Virus recognition in electron microscopy images’, Computer methods and programs in biomedicine, 178, pp. 31–39.
McAuliffe, M.J. et al. (2001) ‘Medical image processing, analysis and visualization in clinical research’, in Proceedings 14th IEEE symposium on computer-based medical systems. CBMS 2001. IEEE, pp. 381–386.
Mishra, A. (2021) ‘Contrast limited adaptive histogram equalization (CLAHE) approach for enhancement of the microstructures of friction stir welded joints’, arXiv preprint arXiv:2109.00886 [Preprint].
Parihar, A.S., Verma, O.P. and Khanna, C. (2017) ‘Fuzzy-contextual contrast enhancement’, IEEE Transactions on Image Processing, 26(4), pp. 1810–1819.
Qidwai, U. and Chen, C. (2009) Digital image processing: an algorithmic approach with MATLAB. Chapman and Hall/CRC.
Rafid Hashim, A., Daway, H.G. and Kareem, H.H. (2022) ‘Single image dehazing by dark channel prior and luminance adjustment’, The Imaging Science Journal, pp. 1–10. Available at: https://doi.org/10.1080/13682199.2022.2141863.
Saravanan, S. and Karthigaivel, R. (2021) ‘A fuzzy and spline based dynamic histogram equalization for contrast enhancement of brain images’, International Journal of Imaging Systems and Technology, 31(2), pp. 802–827.
Setiawan, A.W. et al. (2013) ‘Color retinal image enhancement using CLAHE’, Proceedings - International Conference on ICT for Smart Society 2013: ‘Think Ecosystem Act Convergence’, ICISS 2013, pp. 215–217. Available at: https://doi.org/10.1109/ICTSS.2013.6588092.
Sheer, A.H. and Daway, H.G. (2024) ‘MRI image enhancement using nonlinear mapping and adaptive histogram equalization based segmentation by Otsu’s method’, in AIP Conference Proceedings. AIP Publishing.
Sikder, N. et al. (2024) ‘Heterogeneous virus classification using a functional deep learning model based on transmission electron microscopy images’, Scientific Reports, 14(1), p. 28954.
Sultan, N.H. (2016) ‘Hybrid image denoising using wiener filter with discrete wavelet transform and framelet transform’, Kufa Journal of Engineering, 7(2), pp. 122–133.
Szerlip, N.J. et al. (2007) ‘Real-time imaging of convection-enhanced delivery of viruses and virus-sized particles’, Journal of neurosurgery, 107(3), pp. 560–567.
Thahab, A.T. (2015) ‘Three dimensional dct and temporal secondary clustering based video steganography’, Kufa Journal of Engineering, 6(2), pp. 90–100.
Valentine, R.C. (1962) ‘Contrast enhancement in the electron microscopy of viruses’, in Advances in virus research. Elsevier, pp. 287–318.
Varga, D. (2023) ‘No-reference image quality assessment using the statistics of global and local image features’, Electronics, 12(7), p. 1615.
Wang, J., Li, Y.J. and Yang, K.F. (2021) ‘Retinal fundus image enhancement with image decomposition and visual adaptation’, Computers in Biology and Medicine, 128, p. 104116. Available at: https://doi.org/10.1016/j.compbiomed.2020.104116.
Wang, W. and Chang, F. (2011) ‘A Multi-focus Image Fusion Method Based on Laplacian Pyramid.’, J. Comput., 6(12), pp. 2559–2566.
Zhao, C. et al. (2019) ‘A new approach for medical image enhancement based on luminance-level modulation and gradient modulation’, Biomedical Signal Processing and Control, 48, pp. 189–196.
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