Effect of Incubation Period and Vitamin C on in vitro Sperm Activation in Oligozoospermic infertile Patients by using FertiCult Flushing Medium

Abstract

Objectives: This study was aimed to know effects of Incubation period and vitamin C on sperm for Oligospermic infertile patients parameters by activated at using mixing technique and using FertiCult Flushing Medium.

Methodology: This study was perform on 32 samples, belongs to 26 patients. In this study divided one samples into two parts. The first part activated by FertiCult Flushing Medium and incubation period (30,45,60,75) min while the second part activated by FertiCult Flushing Medium with vitamin C.

Results: The results showed that there was a significant improvement (P<0.05) in the sperm motility percent and sperm grade activity in comparison to the results before activation and the results showed significant improvement (P<0.05) in the sperm motility percent and sperm grade activity in 60 min activation Incubation period comparison to that activation in Incubation period (30,45,75) min. The results showed that there was a significant improvement (P<0.05) in the sperm motility percent, sperm grade activity in activation at using FertiCult Flushing Medium with vitamin C in 60 min Incubation period comparison to its result in activation at used by FertiCult Flushing Medium without vitamin C in 60 min Incubation period.

Conclusion : using 60 min Incubation period a human sperm activation can give a better results than the activation in (30,45,75) min Incubation period. Also, it was found that addition of vitamin C to FertiCult Flushing Medium improve the sperm parameters in Oligospermia.