Effect of Zizphus Plant Extract on The Viability of Hydatid Cysts Protoscolecies In Vitro

Abstract

Objective: The aim of this study to evaluate the effectiveness of the aquatic extract of Zizphus spina Christi, leaves on the viability of the protoscolecies in the larvae stage of Echinococcus granuloses in vitro.

Methodology: Hydatid cysts were collected from the sheep livers in AL-Najaf abattoir and the number of protoscolecies were counting by added (1 ML ) of protoscolecies suspension to (1ML ) of each concentration from plant extract for a different period of time and the percentage of the viability of the protoscolecies was measured by using the Eosin aqueous stain (0.01%) .

Results: The results of the In vitor. study reveal-ed reducing the mean of protoscolecies in 87 to 9 at the sixth day of the experiment with the (10%) concentration and from 89 to zero at the second day with the(30 %) concentration. the cool aquatic extract while the hot aquatic extract the mean reduced from 87 to zero at fourth day with (10%) concentration and from 85 to zero at first day with (30%) concentration.

Conclusion: The extract leaves zizyphus warm and cold aquatic have had a clear impact in reducing of protoscolecies, which is directly proportional to increase concentration and reduce the duration of protoscolecies.

Recommendations: a future study using organic solvents such as methanol to extract the active compounds of the plant zizyphus and study its impact on leaves on the viability of the protoscolecies in the larvae stage of Echinococcus granuloses in vitro.