EFFECTIVENESS OF PHENOL COMPOUND ISOLATED FROM MENTHE SPICATA LEAVES AND NANO – ZINC OXIDE ON ANTIMICROBIAL ACTIVITY
DOI:
https://doi.org/10.36320/ajb/v15.i1.11723Keywords:
Phenol, Menthe spicata, Nano – Zinc Oxide, AntimicrobialAbstract
The aim from this the study effect Phenol compound isolated from Menthe spicata leaves and Nano – Zinc Oxide on Antimicrobial activity antifungal (Candida albicans ) and antibacterial (staphylococcuse aureas and Escherichia coli) , The Menth spicata plant collection from the city of Karbala in 2/9/ 2022 after cleaning and removing soil. As the seed were washed three times with tap water , extraction by using soxhelet apparatus by using methanol (70%) , through 24 hours of and evaporation by rotary evaporation apparatus 40C° for drying, and isolation phenol from Menthe spicata leaves , E. coli and Sta. aureus and C.albicans were obtained from libraries university of karbala , the antimicrobial test against (S.aureas , E.coli and C.albicans) was detected by disc diffusion methods was carried out using phenol extract at concentrations of 0.5, 1, 2, and 13 mg/ml , while Znonanoparticles was performed 0.2, 0.5, 0.8, and 1 mg/ml concentration . indicate the results the inhibition zone of phenol was 17.5 against C.albicans , 13.76 mm against E .coli, 12.87 mm against S. aureus in concentration (0.5) mg/ml. while concentration 1 mg/ml in C.albicans 18.34 , S. aureus 13.98mm , E.coli 14.98mm , while concentration 2mg/ml in C.albicans 19.21mm , S. aureus 14.98mm , E.coli 16.87 mm , concentration 3 mg/ml in C.albicans 21.65mm S. aureus 16.87 mm , E.coli (17.98) mm. While Znonanoparticles' C.albicans inhibition zone was 18.98, , 19.76 mm against E .coli, 20.76 mm against S. aureus in concentration 0.2 mg/ml, while concentration 0.5 mg/ml in C.albicans 19.32 , S. aureus 21.87mm , E.coli 20.54mm , while concentration 0.8mg/ml in C.albicans 21.87mm , S. aureus 22.87 , E.coli 16.87 mm , concentration 1 mg/ml in C.albicans 22.98mm , S. aureus 24.8 mm and E.coli 23.98 mm. The study's finding is that C.albicans was inhibited by phenol compounds at various doses., while the Znonanoparticles with various doses caused the antibacterial activity to be inhibited (S.aureas , E.coli and C.albicans).
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