Molecular detection of some virulence factors of hypervirulent Klebsiella pneumonia that associated with pathogenicity
DOI:
https://doi.org/10.36320/ajb/v15.i2.12008Keywords:
Phenotypic, Klebsiella pneumonia, Pathogenicity, Virulence Factors, Klebsiella pneumonia, Phenotypic DetectionAbstract
Abstract: The study amid to isolation and identification of Hypervirulent K. pneumonia (hvKp) from clinical samples and virulence factors genes was detected. The present study included specimens collected from (Urine, Diabetic Foot, sputum , Liver abbesses , Blood , CSF ,and Semen ,) of patients infected with K. pneumonia in hospitals in Najaf City for both sexes, Male were (34) and Female (47) of age ranged between (10-50) years during the period September to December - 2022.
Eighty (80/100) isolates of K. pneumonia were detected by Morphological, microscopy and biochemical and confirmed by VITEK-2 system as a (hvKp). The result of phenotypic detection of some virulence factors such as biofilm , capsule, sidrophore, Outer membrane Protein(omp) and haemolysis), all isolates were biofilm producers(100%), with three different categories. According to the results of screening virulence genes, BssS (n= 80, 100%) was prevalent among all the clinical isolates, followed by the rmpA (n=22, 27.7%), and magA (n=3 .7%),
Downloads
References
Paczosa, M. K., and Mecsas, J. (2016). Klebsiella Pneumoniae: Going on the Offense With a Strong Defense. Microbiol. Mol. Biol. Rev. 80 (3), 629–661. doi: 10.1128/MMBR.00078-15
Dao, T. T., Liebenthal, D., Tran, T. K., Ngoc Thi Vu, B., Ngoc Thi Nguyen, D., Thi Tran, H. K., et al. (2014). Klebsiella Pneumoniae Oropharyngeal Carriage in Rural and Urban Vietnam and the Effect of Alcohol Consumption. PloS One 9 (3), e91999. doi: 10.1371/journal.pone.0091999
AL-Huchaimi, S. H. K., Jassim, A., AL-Hadad, M. T. S., & AL-Ammar, M. H. (2018). DETECTION OF PATHOGENICITY MARKERS PRODUCED BY PSEUDOMONAS AERUGINOSACAUSING SKIN INFECTION. Plant Archives, 18(1), 621-626.
Martin, R.M & Bachman, M.A (2018). Colonization, Infection, and the Accessory Genome of Klebsiella pneumoniae. Frontiers in cellular and infection microbiology.
Vading M, Nauclér P, Kalin M, Giske CG.(2018). Invasive infection caused by Klebsiella pneumoniae is a disease affecting patients with high comorbidity and associated with high long-term mortality. PLoS One. 2018;13(4):1–13.
Patel, S., H.C. Chauhan, C. Patel, D. Shrimali, B. Patel, I. Prajapati, K. Kala, G. Patel, rajgor, M. and Patel, A. (2017). Isolation and Identification of Klebsiella pneumoniae from Human-Case Report.
Al-Fanharawi Ahmad Hasan. Al-Shamarti Mohammed Jasim Al-Shamarti Al-Janah Haidar Chayad(2022). The Role Of OprD Gene In The Carbapenems Resistance In Pseudomonas Aeruginosa Isolated From Burns Infection In Al-Najaf Province: Al-Kufa University Journal for Biology Vol. 14 No. 3. DOI:https://doi.org/10.36320/ajb/v14.i3.11150.
Wang, X. ; Xie, Y. ; Li, G. ; Liu, J. ; Li, X. ; Tian, L. and Qu, H. (2018). Whole-Genome-Sequencing characterization of bloodstream infectioncausing hypervirulent Klebsiella pneumoniae of capsular serotype K2 and ST374. Virulence, (just-accepted).
Collee, J. G. ; Fraser, A. G. ; Marmion B. P. and Simmon, A. ( 1996 ) . Pactical Medical Microbiology . 14th Ed . Churchill Living Stone , USA Collins, C. H. and Lyne, P. M. Microbiological Methods. 14th Ed., Butterworth and Co. (Pubilisher).
Alsadawi Aqeel A. Alsadawi, Alammar Mahdi, Hamid Mohammad (2022).The Role of TLR-4(896A/G) Gene polymorphisms in patients with Diabetic foot ulcer Al-Kufa University Journal for Biology: Vol. 14 No. 3. DOI: https://doi.org/10.36320/ajb/v14.i3.
AL-Ammar, M. H., & AL-Quraishi, Z. H. O. (2018). PHENOTYPIC DETECTION OF SOME VIRULENCE FACTORS IN SALMONELLA TYPHI CARRIER ASSOCIATED WITH GALL BLADDER CHRONIC INFECTION. Plant Archives, 18(1), 1081-1087.
Sura, A.A. (2015). Study the Effect of Biofilm of Klebsiella pneumoniae Isolated from Different Clinical Sources on Some Immune System Aspects Msc. Thesis, Biology/ Microbiology, collage of science, Al-Mustansiriya.
Lizcano, L. J., Bakkali, F., Ruiz-Larrea, M. B., & Ruiz-Sanz, J. I. (2010). Antioxidant activity and polyphenol content of aqueous extracts from Colombian Amazonian plants with medicinal use. Food Chemistry, 119(4), 1566-1570.
MacFaddin, F., (2000). Biochemical tests for identification of medical bacteria, 3rd ed. Baltimore: Williams & Wilkins.
Al-Ammar, M., & Al Maghathry, A. S. (2017). Molecular detection of antibiotic resistance genes of Acenitobacter bumannii. Pakistan Journal of Biotechnology, 14(4), 777-783.
Doyle, J. and Evans, J.R. (2009)."Klebsiella,". J AccessScience.33: 100-300 .
Aljanaby, A. A.J. and Alhasani, A. H. A. (2016). Virulence factors and antibiotic susceptibility patterns of multidrug resistance Klebsiella pneumoniae isolated from different clinical infections. Afr. J. Microbiol.
Alnasrawy, W. D., & AL-Aammar, M. H. (2021). A Molecular Study with A Comparison of the Odds of Diagnostic Methods For Burkholderia Cepacia Bacteria Isolated from Patients with Diabetic Foot Ulcer. Indian Journal of Forensic Medicine & Toxicology, 15(3), 4603-4609.
AL-Salem L.H. H. and Bunyan A. Ilham(2021).Phenotypic and Genotypic Detection of some virulence factors among Klebsiella pneumonia isolates from different site of infections. Biochem.Cell.Arch. 21(1):1449-1454.
AL-Kraety, I. A. A., & Al-Ammar, M. (2017). relation of class1 integron gene with multi-drug resistance salmonella typi isolates. Pak. J. Biotechnol. Vol, 14(4), 537-541.
[
Hussien, F. H., & AL-Ammar, M. H. (2022). Molecular study of some pathogenic bacteria associated with the diabetes patients. International Journal of Health Sciences, 6829–6838. https://doi.org/10.53730/ijhs.v6ns4.10309.
Deepika, A and Meena, A. (2015). Correlation between biofilm production and Antibiotic Resistance Pattern in Uropathogenic Escherichia coli in tertiary care hospital in southern rajasthan, Indian International Journal of Current Microbiology and Applied Science, 4(7): 640-646.
Al-Sadawi, A. A., & Al-Ammar, M. (2022). Association between Genotypic and Phenotypic Formation of Biofilm in Pseudomonas aeruginosa Isolated from Diabetic Foot Patients. HIV Nursing, 22(2), 199-203.
Ariadnna, C.C.; Verónica E.K.; Karina E.M.; Sara A.O.; Sarbelio. M.E.; Alicia, G.E.; Elizabeth, F.R.; Edgar, O. L. V. and Juan, X.C. (2014). Pathogenic determinants of clinical Klebsiella pneumoniae strains associated with their persistence in the hospital environment. Boletín Médico Del Hospital Infantil de México ,71(1):15-24.
Lee, I. R., Molton, J. S., Wyres, K. L., Gorrie, C., Wong, J., Hoh, C. H., et al. (2020). Differential host susceptibility and bacterial virulence factors driving Klebsiella liver abscess in an ethnically diverse population. Sci. Rep. 6:29316.
AL-Quraishi, Z. H. O., & AL-Amm, P. H. (2018). Genotypic Study of Some Virulence Factors in Salmonella typhi Carrier Associated with Gall Bladder Chronic Infection.. International Journal of Pharmaceutical Quality Assurance 2018; 9(2); 132-137 doi: 10.25258/ijpqa.v9i2.13635.
Pegah Shakib1;2, Morovvat Taheri Kalani3,Rashid Ramazanzadeh1;2;4,Amjad Ahmadi1;2 and Samaneh Rouhi1;2 (2018).Molecular detection of virulence genes in Klebsiella Pneumoniae clinical isolates from Kurdistan Province, Iran Biomedical Research and Therapy 2018, 5(8): 2581-2589
M. Abd Asada , M., & Aziz Mahal Al-amri, N. (2021). Molecular identification and Virulence factors of Pseudomonas aeruginosa isolated from operation hall. Al-Kufa University Journal for Biology, 13(2), 39–46. https://doi.org/10.36320/ajb/v13.i2.11758.
Abdul Redha Aziz, R., & Alaa Saud, S. (2021). PCR detection of quorum sensing autoinducer (AI) type one among gram negative bacteria. Al-Kufa University Journal for Biology, 13(1), 17–25. https://doi.org/10.36320/ajb/v13.i1.8136
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Emad kareem hanoon, Mahdi Hussain AL-Aammar, Fatemeh Sobhani monfared
This work is licensed under a Creative Commons Attribution 4.0 International License.
which allows users to copy, create extracts, abstracts, and new works from the Article, alter and revise the Article, and make commercial use of the Article (including reuse and/or resale of the Article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made and the licensor is not represented as endorsing the use made of the work.
